Culture of Animal Cells A Manual of Basic Technique and Specialized Applications

Download or Read online Culture of Animal Cells A Manual of Basic Technique and Specialized Applications full book in PDF, ePub and kindle by R. Ian Freshney and published by John Wiley & Sons which was released on 05 January 2016 with total pages 696. We cannot guarantee that Culture of Animal Cells A Manual of Basic Technique and Specialized Applications book is available in the library.

Culture of Animal Cells   A Manual of Basic Technique and Specialized Applications
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Publisher : John Wiley & Sons
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ISBN : 1118873653
Pages : 696 pages
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Download or Read Online Culture of Animal Cells A Manual of Basic Technique and Specialized Applications in PDF, Epub and Kindle

This is the 7th edition of a textbook first published in 1983. It aims to provide basic instruction in the basic procedures of cell culture for newcomers to the field, including aseptic technique, safety and regulatory issues, equipment and materials, media preparation and sterilization, primary culture, propagated cell lines, characterization and authentication, contamination, cryopreservation, and quantitation. There are also a number of specialized protocols some of which have general interest, e.g. cell cloning, 3D culture, scale-up, STR profiling, and some with a with more limited readership, e.g. culture of some specialized cells. Some specialized protocols will be retained in the printed copy but others will be presented in electronic form only, depending on the anticipated readership. A number of minireviews, some by the author external review and some by invited authors will be added to give an overview of the applications of cell culture. New approaches and procedures have become available and new issues have arisen which require sections of the book to be updated. The increasing diversity of the applications of cell culture also need a revision of how certain topics are presented. The proliferation of specialized techniques requires that some of these now be presented online to avoid a further increase in size of the book. In addition the introduction of new topics requires that some of these be presented in mini-review form. Three reviewing editors have been appointed to advise on recent developments and trends and this will help to reshape the book in line with cutrrent demand. Some new features: There will be a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling (existing) and barcoding (new). Some specialized protocols, e.g. much of chapters 22, 23, and 27, will be removed and made available online (free to those who have purchased the print copy). This edition will focus more on more generally used techiniques and make other less used techniques available online. New mini-reviews will give insight into newer applications. More emphasis will be given to authentication and problems of misidentification. Illustrations will be updated as required.

Culture of Animal Cells   A Manual of Basic Technique and Specialized Applications

This is the 7th edition of a textbook first published in 1983. It aims to provide basic instruction in the basic procedures of cell culture for newcomers to the field, including aseptic technique, safety and regulatory issues, equipment and materials, media preparation and sterilization, primary culture, propagated cell lines, characterization and

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Culture of Animal Cells

This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in

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Freshney s Culture of Animal Cells

FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment,

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Animal Cell Culture

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It

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Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the

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Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for

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Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and

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Animal Cell Culture

Animal Cell Culture: A Practical Approach has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the series. This new edition takes account of the progress that has been made in the field. Although the basic principles remain the same,significant advances have

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